AGROSLUОBY OLOMOUC, s.r.o.AGROSLUОBY OLOMOUC, s.r.o

VALIDACE METODY
 

AGROS ELISA KIT



 Laborat/x rщstnvэch regtlбtory UP OloMouc &

V O$ommubi 16.5.2005

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Popis AGROS ELISA jitu.

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 Analytickэ princip.

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Regresnн koeficient R2 = 0,9975
SD smмrnice B = 0,00961

 

         Hodnocenб metoda byla dбle srovnбna s ovмшenou metodou RIA pro stanovenн progesteronu. Progesteron byl souиasnм stanoven ve tшiceti neznбmэch vzorcнch pomocн AGROS ELISA kitu v Laboratoшi rщstovэch regulбtorщ a pomocн RIA kitu dodбvanйho firmou Immunotech v Imunoanalytickй laboratoшi Kliniky nukleбrni medicнny Fakultnн nemocnice Olomouc. Oba soubory namмшenэch hodnot byly nбslednм srovnбny pomocн statistickйho programu NCSS97 v pбrovйm T-Testu, kterэ potvrdil vzбjemnou shodu souborщ.


Paired T-Test Report

Page/Date/Time                   1    6.5.2005 13:29:44

Database                           

Variable                              X1 = C1, X2 = C2

 

Descriptive Statistics Section

                                                                        Standard          Standard          95% LCL           95% UCL

Variable                  Count        Mean               Deviation         Error                of Mean           of Mean

C1                           30             6,888333           5,568537           1,016671           4,809008           8,967659

C2                           30             7,261667           6,36519             1,162119           4,884866           9,638468

Difference                 30             -0,3733333        1,784113           0,3257329         -1,039532          0,2928652

T for Confidence Limits = 2,0452

 

Tests of Assumptions about Differences Section

 

Assumption                                       Value             Probability    Decision(5%)

Skewness Normality                            -0,6922           0,488823        Cannot reject normality

Kurtosis Normality                               -0,6793           0,496942        Cannot reject normality

Omnibus Normality                              0,9406            0,624822        Cannot reject normality

Correlation Coefficient                          0,964051

 

T-Test For Difference Between Means Section

 

Alternative                                                              Prob              Decision        Power           Power

Hypothesis                                         T-Value          Level             (5%)                (Alpha=.05)    (Alpha=.01)

C1-C2<>0                                           -1,1461           0,261110        Accept Ho    0,198312        0,067750

C1-C2<0                                             -1,1461           0,130555        Accept Ho    0,299640        0,108820

C1-C2>0                                             -1,1461           0,869445        Accept Ho    0,002848        0,000311

 

Nonparametric Tests Section

 

Quantile (Sign) Test

 

Hypothesized                                         Number         Number         Prob              Prob              Prob

Value                             Quantile           Lower            Higher           Lower            Higher           Both

0                                     0,5                    13                  17                  0,292332         0,819203        0,584665

 

Wilcoxon Signed-Rank Test for Difference in Medians

 

W                                   Mean               Std Dev         Number         Number Sets  Multiplicity

Sum Ranks                     of W                 of W              of Zeros         of Ties           Factor